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Developing a common bean core collection suitable for association mapping studies Genet. Mol. Biol.
Perseguini,Juliana Morini Küpper Cardoso; Silva,Gliciane Micaele Borges; Rosa,João Ricardo Bachega Feijó; Gazaffi,Rodrigo; Marçal,Jéssica Fernanda; Carbonell,Sérgio Augusto Morais; Chiorato,Alisson Fernando; Zucchi,Maria Imaculada; Garcia,Antonio Augusto Franco; Benchimol-Reis,Luciana Lasry.
Because of the continuous introduction of germplasm from abroad, some collections have a high number of accessions, making it difficult to explore the genetic variability present in a germplasm bank for conservation and breeding purposes. Therefore, the aim of this study was to quantify and analyze the structure of genetic variability among 500 common bean accessions to construct a core collection. A total of 58 SSRs were used for this purpose. The polymorphism information content (PIC) in the 180 common bean accessions selected to compose the core collection ranged from 0.17 to 0.86, and the discriminatory power (DP) ranged from 0.21 to 0.90. The 500 accessions were clustered into 15 distinct groups and the 180 accessions into four distinct groups in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular markers; Genetic diversity; Genetic structure; Microsatellites; Phaseolus vulgaris L.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100067
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Linkage and mapping of quantitative trait loci associated with angular leaf spot and powdery mildew resistance in common beans Genet. Mol. Biol.
Bassi,Denis; Briñez,Boris; Rosa,Juliana Santa; Oblessuc,Paula Rodrigues; Almeida,Caléo Panhoca de; Nucci,Stella Maris; Silva,Larissa Chariel Domingos da; Chiorato,Alisson Fernando; Vianello,Rosana Pereira; Camargo,Luis Eduardo Aranha; Blair,Matthew Wohlgemuth; Benchimol-Reis,Luciana Lasry.
Abstract Angular leaf spot (ALS) and powdery mildew (PWM) are two important fungi diseases causing significant yield losses in common beans. In this study, a new genetic linkage map was constructed using single sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs), in a segregating population derived from the AND 277 x SEA 5 cross, with 105 recombinant inbred lines. Phenotypic evaluations were performed in the greenhouse to identify quantitative trait loci (QTLs) associated with resistance by means of the composite interval mapping analysis. Four QTLs were identified for ALS resistance. The QTL ALS11AS, linked on the SNP BAR 5054, mapped on chromosome Pv11, showed the greatest effect (R2 = 26.5%) on ALS phenotypic variance. For PWM resistance,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudocercospora griseola; Erysiphe polygoni; Quantitative inheritance; SSRs; SNPs.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100109
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Mapping QTLs for drought tolerance in a SEA 5 x AND 277 common bean cross with SSRs and SNP markers Genet. Mol. Biol.
Briñez,Boris; Perseguini,Juliana Morini Küpper Cardoso; Rosa,Juliana Santa; Bassi,Denis; Gonçalves,João Guilherme Ribeiro; Almeida,Caléo; Paulino,Jean Fausto de Carvalho; Blair,Matthew Ward; Chioratto,Alisson Fernando; Carbonell,Sérgio Augusto Morais; Valdisser,Paula Arielle Mendes Ribeiro; Vianello,Rosana Pereira; Benchimol-Reis,Luciana Lasry.
Abstract The common bean is characterized by high sensitivity to drought and low productivity. Breeding for drought resistance in this species involves genes of different genetic groups. In this work, we used a SEA 5 x AND 277 cross to map quantitative trait loci associated with drought tolerance in order to assess the factors that determine the magnitude of drought response in common beans. A total of 438 polymorphic markers were used to genotype the F8 mapping population. Phenotyping was done in two greenhouses, one used to simulate drought and the other to simulate irrigated conditions. Fourteen traits associated with drought tolerance were measured to identify the quantitative trait loci (QTLs). The map was constructed with 331 markers that covered all...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abiotic stress; Interpopulation gene-pool; Molecular markers; QTL mapping; Water deficit.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500813
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